Major modes of using cryopreserved amniotic membrane
Cryopreserved amniotic membrane (Bio-Tissue, Miami, FL www.biotissue.com) can be used either as a Permanent Graft, a Biologic Bandage, or both.
| |
Permanent Graft |
Biologic Bandage |
| What biologic action(s) are primarily delivered? |
Anti-inflammatory, anti-scarring, anti-angiogenic, and growth promotion |
Anti-inflammatory and growth promotion |
| Where does the epithelialization occur? |
Over the membrane |
Under the membrane |
| Will the membrane be integrated into the host tissue? |
Yes
(either conjunctiva or cornea) |
No |
| Will the membrane be removed/dissolve? |
No |
Yes. After healing the tissue will dissolve or be removed. Rapid dissolution in less than one week suggests an "exposure" problem |
| How many layers are needed? |
One or multiple layers to fill in stromal defects of any shape |
One |
| Does the orientation matter? |
Yes
(stromal/sticky side down for the top layer) |
No |
| Do I need to use sutures? |
Fibrin glue or sutures can be used to secure the graft (if multiple layers are used, the top layer must be sutured) |
10-0 nylon sutures by purse-string perilimbal or running otherwise |
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Differences in cryopreserved amniotic membrane delivery mechanisms >>
If you have additional surgical questions after viewing this information, contact OSREF’s Research Director, Scheffer C.G. Tseng, MD, PhD, by e-mail at stseng@ocularsurface.comor by phone at 305-274-1299.
Financial Interest Disclosure: Dr. Tseng and his family are more than 5% shareholders in TissueTech, Inc. and Bio-Tissue, Inc. which currently distributes AMNIOGRAFT® and PROKERA®
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