Amniotic Membrane Transplantation for Limbal Stem Cell Deficiency


The maintenance of a healthy functional corneal epithelium is provided by a unique subpopulation of stem cells located in the limbal region1. When limbal epithelial stem cells are destroyed or become dysfunctional, a pathological state known as limbal stem cell deficiency (LSCD) manifests. The hallmark of LSCD is the conjunctivalization of the cornea2, and is frequently associated with superficial vascularization and compromised corneal surface3.

LSCD can be found with a number of corneal diseases such as chemical burns, Stevens Johnson syndrome, aniridia, peripheral keratitis, severe limbitis, etc (for more details see Table 1). Patients with LSCD suffer from a severe loss of vision and annoying photophobia, and cannot be corrected by conventional PKP. Therefore, it is important to accurately diagnose LSCD because erroneous diagnosis may subject the patient to unnecessary surgeries. Correct diagnosis, which may require the use of impression cytology, will lead to reconstruction with limbal stem cell transplantation.

[Expand all]

  • Corneal diseases with Limbal SC Deficiency
  • Partial LSCD
  • Total LSCD: Unilateral
  • Total LSCD: Bilateral
  • Other Considerations in Treating LSCD
  • Supplies for Treating LSCD with Amniotic Membrane Transplantation
  • References


  • AMT procedure for partial LSCD Video

  • Procedure for AMT with CLAU Video

  • Procedure for AMT & KLAL Video

If you have additional surgical questions after viewing this information, contact OSREF's Research Director, Scheffer C.G. Tseng, MD, PhD, by e-mail at or by phone at 305-274-1299.

Videos edited by: Scheffer C.G. Tseng, MD, PhD, Hosam Sheha, MD, PhD, Ahmad Kheirkhah, MD, Antonio Elizondo, MD, Victoria Casas, MD

Note: The videos are for peer discussion purposes and the creators are not participating in commercial promotion of any product.

Financial Interest Disclosure: Dr. Tseng and his family are more than 5% shareholders in TissueTech, Inc. and Bio-Tissue, Inc. which currently distributes AMNIOGRAFT® and PROKERA™

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